SpectraMax® M2 SpectraMax® M2e Multi-Mode Microplate Readers User Guide 0112-0102 E October 2018 SpectraMax M2 and M2e Microplate Reader User. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. Add to Cart. S. We suggest Corning 384-well plates Cat. BMG LABTECH. Tecan’s. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. White plates reflect the light, enabling even low levels of Luminescence to be detected. For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. 5°C at 37°C. Using standard fluorescence optimization can take hours or days to determine the best wavelengths to use for excitation. The SpectraMax. Table 1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Fluorescence intensity values increased over time, but similar EC 50 values of 263 nM and 295 nM were obtained for assay incubation times ranging from one. You should use solid white plates for top Time- Resolved Fluorescence reads, and white plates with clear bottoms for bottom reads. Add to Cart. This user guide provides detailed instructions, troubleshooting tips and technical specifications for these instruments. 67. すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. 1 x 38. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. spr SpectraMax iD5 SpectraTest ABS2 v1. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. Microplate Reader Shelf. Read the microplate Step 1: Place the microplate in the reader. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. 5. The plate is set up for reading and images are acquired according to specified parameters. E Models. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. 1) Select the correct plate and the wells that you want to measure. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. com 10. These measurements can include parameters such as absorbance, fluorescence intensity and. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. Transcreener ® ADP. 0 out of 5 $ 3,895. Linear, Orbital Slow, and Orbital Fast. The flexibility of a high end multimode plate reader with environmental control. SpectraMax M2 and use thereof is covered by issued (U. SpectraMax M2 Readers provide for easy conversion and optimization of very-low-throughput to medium-high-throughput assays, faster, more precise results and reagent savings. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. 0 Concentration Dynamic Range 105 RFU MT oltage ignal Adjustable PMT gain High. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. CO 2 & O 2 control. MPN: 50505. Microplate Reader Shelf. Absorbance cuvette port. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. SpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader Absorbance ü ü ü ü Fluorescence Intensity ü ü ü ü Luminescence ü ü ü ü Time-Resolved Fluorescence ü ü ü Fluorescence Polarization ü ü HTRF ü Upgrade Options TRF, HTRF, FP HTRF, FP HTRF N/A Plate Formats 6-, 12-, 24-, 48-, 96-, 384. Add to Cart. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. 315 can't find zero order. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Add to Cart. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. Suggested plates for best data: Fluorescence-top read; Solid Black flat-bottom for Excitations above 350 nm;. 04 to 3. Uses dual. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Select the lab user profile. 0 × 103 to 2. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideELISA Plate Readers / Microplate Readers. td. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. Dose-dependent enzyme essay Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. Third this recommendation. The following general steps are taken with our absorbance and fluorescence readers. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. spr * SpectraMax i3(x) SpectraTest ABS2 v1. 3) At the bottom of the strip, click on "Show. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). The optical systems use two scanning monochromators soUnlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 25 to 3. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. 2 only) SpectraMax M-series Microplate readers. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Add to Cart. 0 out of 5 $ 14,499 $ 13,995-13%. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. A microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Perkin Elmer Envision. 4. , contact. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Biotek Synergy HT Microplate Reader Multi-Mode. S. Factors that contribute to an instrument’s sensitivity, or limit of detection, include background noise from the detector and electronics, the type of detector that is used, and the instrument’s configuration and. PerkinElmer’s new EnSight Multimode Plate Reader reads label-free Corning® Epic® technology (in addition to standard labeling technologies) and is appropriate when multiple assay types are more important than speed. Optimized for fluorescence microplate readers. Email. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Microplate Reader, Multimode. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. The Spectramax i3 is a muti-mode plate reader used for measuring absorbance and fluorescence in microtiter plates. The drawing shows a standard microplate of 15 mm height covered with a lid. Figure 2. The emitted• Fluorescence Intensity (FI) • Luminescence (Lum) • Time-Resolved Fluorescence (TRF) • Fluorescence Polarization (FP) The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. 3: The cuvette chamber. fluorescence intensity (Figure 6). 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader andMicroplate Readers. Standard features include a cuvette port,. Microplate Formats: 6, 12, 24, 48, 96, 384. 312 gain calibration failed. A). D 4/21/06 9:53:29 AM . Reads various kinds of 96-wells plates, and is equipped with shaking function. The calculated LOQ values were 0. Plate reader set to 50 reads. 2. 25 to 3. 0077 OD 0. Perform the following steps to install the drivers: Start > Control Panel > System. #4513: Plate Reader: A multi-detection microplate reader configured to measure the output of the UDP tracer is required. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Main Menu Toggle. Published Dec 16, 2020 | Updated Dec 16, 2020. Shop now. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. The plate is set up for reading and images are acquired according to specified parameters. For a higher . 2. Selecting your. Microplate readers’ standard detection modes are. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Data from slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 67. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). Incubation. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. Molecular Devices SpectraMax M2 Multimode Microplate Reader w/ Laptop & Software. Plate reader set to 50 reads. 3) At the bottom of the strip, click on "Show. Add to Cart. 25 to 3. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. Wavelength optimization. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. 4. BioTek Synergy 2. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. $ 2,499. Customer Satisfaction is. The chamber. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. On microplate readers,. Endpoint, kinetic, spectrum. Offer. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microp late Readers by. ABS1 Absorbance Plates, FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The GloMax® line includes high-performance plate readers with a range of detection options. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. free tracer decreases, resulting in an overall increase in RFU values. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Five preferred detection modes. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. SpectraMax ® M2/M2e: Not Capable: Validated:. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 9000-0756. Important things to consider when purchasing a multimode microplate reader. Microplate Readers Molecular Devices Spectramax M3 Multi Mode Microplate Reader. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. 7. Temperature control range from Ambient +4º to 50ºC. 1. Molecular Devices SpectraMax M2. The chamber. Molecular Devices i3x. 306 plate air cal fail,low light. Microplate readers’ standard detection modes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Model: SpectraMax M2. 303 unable to cal dark current. After the plate is read, data will appear in the plate. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. D 3 1. 4. spr SpectraMax Plus 384 SpectraTest ABS2 v1. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. SpectraMax Microplate Readers. refurbished. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. . With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Published Dec 16, 2020 | Updated Dec 16, 2020. ~ Bio-Tek ELx808 Microplate Reader $2,795. Python 3. Its dual light sources, a xenon lamp and LEDs, illuminates samples with incomparable signal strength and superior sensitivity. Category 2 and 3 microplate readers use gradient heating as well. An approach to gain setting that I like to use is to set the gain based on the sample with the most fluorescence in order to get about 30-50% of the maximal signal before the detector saturates. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. For a higher degree of automation, the Automation. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, SpectraMax M4 reader, SpectraMax M3. Microplate Readers. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Stage and carriage are homed and position is tested and verified. 0 out of 5 $ 9,995. For example, microplates containing different samp les can be read using the same or The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). SpectraMax M2 and use thereof is covered by issued (U. Both the SpectraMax M5 and the Gemini EM are monochromator-based, microplate readers. When the user applies a set temperature of 37°C, the top is heated to 37. Bottom Monochromator: Fluorescein 2. Usage/Application: Lab. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. 1 New Reader Support 1. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. When connecting for the first time to either the SpectraMax L reader or StakMax system. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 1. Molecular Devices SpectraMax M2 ; Molecular Devices. As new assays are developed or yourFor TRF assays, you will need a fluorescence plate reader equipped with a time-resolved fluorometry option and a 330 nm excitation filter and emission filters capable of detecting 647 nm (620/40) and 665 nm (665/ 7. For paper-based measurements, we used the Milenia HybriDetect 1 (TwistDx) lateral flow system, as per the manufacturer's. Item Description FrontPanelOperation SinglewavelengthAbsorbance,%Transmittance,Fluorescence readingofthecuvette(ortesttube) UsingSoftMaxProSoftware ExpressdataasAbsorbance. 100 readings per well gives optimal Z’ values for this assay. Brand: MOLECULAR DEVICES. 209, but in 96-well plate was 0. 0 out of 5 $ 7,699. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. The SpectraMax Paradigm Detection Cartridge design utilizes an energy intensity optimization technique that allows the user to read up to seven logs of sample concentration in a single pass of a plate without any manual intervention. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Adjusting fluorescence gain on microplate readers. Compact and tunable absorbance microplate readers DATA SHEET. When reading optical density at wavelengths below 340 nm, special UVtransparent,. The pre-configured protocols can be selected for each assay and. 47 and 0. 0 out of 5 $ 9,995 $ 9,495. 2. Reader Documentation Version No. Plate reader settings for NanoOrange assay. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Fully tested with validations and warranty. Used. 00 Free shippingResults show a linear relationship between fluorescence intensity and cell number (Fig. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax fluorescence microplate reader with SoftMax® Pro Software, is a quick, sensitive detection method for proteins. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. NanoQuant plate. 1. STAKMAX. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. The fluorescence intensity of MitoTracker® Red CM-H2XRos was measured with a fluorescence plate reader at excitation and. Our absorbance plate readers feature our PathCheck Sensor. We would like to show you a description here but the site won’t allow us. Our lab has a BMG labtech Pherastar FS, it is easy to use and we have never had any issue with it. . The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SpectraMax ID5 Plate Reader (Molecular Devices) Description Internal price (Cornell and Cornell affiliates) External Price; SpectraMax ID5 Plate Reader Use: $20/hr:Reads a 96-well plate in 15 s, a 384-well plate in. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The Cut-off filter for this pair is showing 530nm in this machine. Self Service Available. Similar settings will work for other SpectraMax readers with fluorescence detection. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. SpectraMax Quant dsDNA Assay Kits. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. 020 OD 2. 9000-0756. Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. It is particularly powerful when monitoring fast kinetic assays in a high. Page 28 Chapter 5: Read Modes and Read Types 1. You should determine the expected mean OD value by reading a clean water-filled plate from each new lot. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. g. WinKQCL ® Endotoxin Detection and Analysis Software Version 6 Features. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a. Tecan’s. 1. S. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Molecular devices SpectraMax M5 Pdf User Manuals. Using a black. Auto-calibration and self-diagnostics. Reviews. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Step 2: Click SoftMax Pro’s Read button. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. 0 out of 5 $ 2,999. Danaher Inc spectramax m2 fluorescence microplate reader Spectramax M2 Fluorescence Microplate Reader, supplied by Danaher Inc, used in various techniques. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE Summary of Contents for Molecular Devices SpectraMax M2. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Plate background OD values of disposable UV microplates vary slightly between lots. 2. I understand the OD measurement in a plate reader varies with the volume of the sample measured. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax Microplate Readers. . It can measure fluorecence from the top or bottom of a plate with a range of 250 - 850 nm. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. Perform the scan and note the wavelength that gives maximum emission. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Appendix A. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. U. Microplate Reader Shelf. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. SpectraMax Glo Steady-Luc Reporter Assay Kit. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 5). The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The IC50s by cell rounding and LysoTracker assays were 0.